Year: 2017 | Month: June | Volume 7 | Issue 1
Evaluation of Antioxidant and Antimicrobial Activity of Rhododendron arboreum Flowers Extract
Abstract:
Ethanolic extract of Rhododendron arboreum flowers was analyzed for antioxidant and antimicrobial activity. Antioxidant activity was determined by DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (Ferric reducing antioxidant power) assays. The antioxidant activity of flowers extract for DPPH assay was 134.1±2.34 mM TE/g and for FRAP assay was 140.6±2.76 mM TE/g. The antimicrobial activity was measured by agar well diffusion method against six different bacterial species out of which; three were Gram-positive and three were Gram-negative. The minimum inhibitory concentration (MIC) and the minimum bacteriocidal concentration (MBC) were also determined for each pathogen using a 96-well microtiter plate method. The results reported in mg/ml, indicated that all the bacterial strains (Gram-positive and Gram-negative) were effectively inhibited by the ethanolic extract of Rhododendron flowers. E.coli exhibited highest sensitivity to flowers extracts with maximum zone of inhibition (17mm) at extract concentration 50mg/ml and Bacillus cereus and Bacillus subtilis showed minimum inhibition (8mm each) at extract concentration of 25mg/ml. Highest MIC was observed against E.coli (1 mg/ml) and Shigella flexneri (1 mg/ml).
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